Microscopes in Development

dual view line scanning confocal
General Overview:
A custom-built line scanning confocal microscope with two opposing views that is capable of imaging samples up to several millimeters in length at high resolution and fast speed.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Confocal
Microscope Stand:
4-pi configuration custom designed
Features:
Capable of imaging large areas and volumes rapidly with high NA objective lenses.
Simultaneous two-sided illumination and detection to increase volumetric speed and obtain sample information that may be occluded from a single view.
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter 405/488/561/640 lasers. A filter wheel with an individual band bass filter for each laser excitation wavelength.
Detector:
Camera
Imaging lenses:
25x, 1.1 NA Water Immersion
40x, 1.25 NA Silicone
60x, 1.4 NA Oil
100x, 1.35 NA Silicone
100x, 1.49 NA Oil
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Custom designed in Matlab
CO2:
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Convention sample mounting approaches. Glass Bottom Petridish
Location - Building and Room:
Lillie 219
Contact Person:
Total Internal Reflection Fluorescence (TIRF) Microscope
General Overview:
Home-built TIRF Microscope capable of illuminating samples in a ring-TIR mode or a single sided TIR.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Widefield/TIRF
Microscope Stand:
Inverted
Features:
Epi illumination or TIR illumination
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter 405/488/561/640 lasers. Individual emission filters can be added manually.
Detector:
Camera
Imaging lenses:
100x, 1.49 NA TIRF lens
Microscope Control software:
Custom designed Graphical User Interface in Matlab and u-Manager
Image Analysis Software:
CO2:
No
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Conventional sample mounting.
Location - Building and Room:
Lillie 219
Hourly Rate for Use:
$25.00
Contact Person:
General Overview:
A home built dual-view SPIM system using multi-immersion lenses for imaging clear brain tissues. This system is also suitable for imaging samples in water/sea water.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Lightsheet
Microscope Stand:
Custom designed frame
Features:
Suitable for imaging large samples at high volumetric speed.
Laser Wavelengths:
405 nm, 488 nm, 561 nm, 640 nm
Excitation/Emission Filters:
Quad band filter for 405/488/561/640 lasers.
Detector:
Camera
Imaging lenses:
14x, 0.4 NA Multi-immersion lenses
23x, 0.7 NA Multi-immersion lenses
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Fiji/Matlab
CO2:
No
Humidity:
No
Light Tight:
No
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room:
Lillie 219
Contact Person:
Anisotropy Imaging System
General Overview:
This system facilitates illuminating samples using a linearly polarized light in Epi or TIRF mode and detects fluorescence emission in parallel and perpendicular directions using a single sCMOS camera.Anisotropy imaging can provide information about protein-protein interaction or protein clustering.
Commercial or Pre-commercial:
Pre-Commercial
Which Facility:
Imaging Innovation Initiative
Imaging Mode:
Optical
If Optical:
Widefield/TIRF
Microscope Stand:
Inverted
Features:
405 nm, 488 nm, 561 nm, 640 nm
Laser Wavelengths:
A single band pass filter appropriate for the illumination.
Detector:
Camera
Imaging lenses:
100x, 1.49 NA Oil TIRF lens
60x, 1.4 NA Oil lens
Microscope Control software:
Custom designed Graphical User Interface in Matlab
Image Analysis Software:
Custom designed Image Analysis Graphical User Interface in Matlab
CO2:
No
Humidity:
No
Light Tight:
Yes
Sample mounting requirements/constraints:
Conventional sample mounting
Location - Building and Room:
Lillie 219
Contact Person: