Back to Resources & Research Facilities The National Xenopus Resource Overview MBLthe NXR Ordering and Pricing Animal Care Mutant Frog Stocks Transgenic Tropicalis Transgenic Laevis Wild Type Frog Stocks Research Facility Resource Workshops and Meetings Contact The NXR Home Groundbreaking Research at the Ůֱ Resources & Research Facilities The National Xenopus Resource Transgenic Tropicalis Xtr.Et(CAGGS-SB10;cryga:RFP)622EMead NXR_1002 Description: (622EHoppers)SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 622E donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)7M8FMead NXR_1003 Description: (7M8FHoppers)SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 7M and 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)7MMead NXR_1004 Description: (7MHoppers)SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 7M donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Et(CAGGS-SB10;cryga:RFP)8FMead NXR_1005 Description: (8FHoppers)SB10 transposase expression from CAGGS promoter/enhancer, γ-cry driving RFP. 8F donor loci. Double transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Xtr.Tg(CMV:memGFP)NXR NXR_1007 Description:(CMVmemGFP) CMV driving ubiquitous expression of membrane GFP Reference: Custom linegenerated by the NXR. Xtr.Tg(eef1a1:GFP)Krieg NXR_1008 Description:(Ef1a-GFP) Eef1a1 driving GFP. Widespread GFP expression apparent from mid-gastrula on. ڱԳ: Xtr.Et(gata1:GFP)mysMead NXR_1012 Description: (mTol2G1GFP-M4msy) GATA-1 promoter driving GFP, mister stripey integration into the apelin receptor gene. GFP expression in red blood cells and venous vasculature. Reference: Mead Lab;. Xtr.Et(krt8:GAL4-VP16,UAS:GFP)Mead NXR_1014 Description: (mTol2k8GAL4 VP16 UASGFP) Keratin 8 minimal promoter driving expression of GAL4VP16 fusion with UAS GFP reporter gene. Made using the miniTol2 transposon. Reference: Mead Lab; Xtr.Tg(otx2:GFP)Kellr NXR_1020 Description: (Otx2-GFP) Otx2 driving GFP. GFP expression of forebrain and midbrain ڱԳ: Xtr.Tg(pax6:GFP;cryga:RFP;actc1:RFP)Grngr NXR_1021 Description: (Pax6-GFP:xcryFRP:CardiacActin-RFP) Coinjection of pax6 driving GFP, γ-cry driving RFP, and actc1 driving RFP. GFP expression in eyes, brain, and spinal cord. RFP expression in lenses and muscle. ڱԳ: Xtr.Et(CAGGS-SB10;cryga:RFP; CAGGS:GFP)622EMead NXR_1025 Description:(pT2BGFP-622E) Widespread GFP fluorescence: outcross of the ‘hopper’ frogs will result in remobilization of the substate transposon and re-integration elsewhere in the genome resulting in expression pattern and/or intensity of GFP expression. Reference: Mead Lab; Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)6MMead NXR_1026 Description: (pT2BGFP-6M) Widespread GFP fluorescence. Reference: Mead Lab; Xtr.Et(CAGGS-SB10,cryga:RFP; CAGGS:GFP)8FMead NXR_1028 Description: (pT2BGFP-8F) LDouble transgenic frogs harboring substrate transposon and transposase enzyme transgenes; outcross of these animals with wild type frogs yields tadpoles with novel re-integration events. Reference: Mead Lab; Xtr.Et(CAGGS-SB10;cryga:RFP)Mead NXR_1034 Description: (SB10enzyme) SB10 transposase expression from CAGGS promoter/enhancer,γ-cry driving RFP. Reference: Mead Xtr.Et(eef1a1:GFP)Hbr,Cen,Fol,BruMead NXR_1037 Description: (Tol2X1G-12MHbrCenFolBru)Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Reference: Mead Xtr.Et(eef1a1:GFP)folMead NXR_1038 Description: (Tol2X1G-12Mfol)Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Fol integration into 5′ UTR of MTHFD1L gene. ڱԳ: Mead Lab; Xtr.Et(eef1a1:GFP)cenMead NXR_1040 Description:(Tol2X1G-Mcen) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Cen expressed in forebrain. Reference: Mead Lab; Xtr.Et(eef1a1:GFP)grbMead NXR_1042 Description:(Tol2X1G-12Mgrb) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Garibaldi (grb) integration into 3′ UTR of smad7 gene. Reference: Mead Lab; Xtr.Et(eef1a1:GFP)hbrMead NXR_1043 Description:(Tol2X1G-12Mhbr) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Expression in cartilage and cardiac outflow tract. Reference: Mead Lab; Xtr.Et(eef1a1:GFP)johMead NXR_1044 Description: (Tol2X1G-12Mjoh) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Joh expressed in kidney, integration near HNF1B gene. ڱԳ: Mead Lab; Xtr.Et(eef1a1:GFP)psvbiMead NXR_1046 Description: (Tol2X1G-16Fpsvbi)Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. Medial somite and ventral blood island expression. Integration likely to be in repeart regiong of MLL2 gene. Reference: Mead Xtr.Et(eef1a1:GFP)16FMead NXR_1047 Description:(Tol2X1G-16Fubiq) Tol2 transposon harboring a Xenopus ef1a promoter driving expression of GFP. 16F fubiq Reference: Mead Lab; Xtr.Tg(cryga:GFP)Grngr NXR_1049 Description: (xCrys-GFP) GFP expression in the lens. Reference: Grainger. Xtr.Tg(CMV:Aequorin,GFP)NXR NXR_1070 Description: (CMV-Aequorin-GFP) Reference: Custom line generatedby NXR for Andrew Miller. Xtr.Tg(tubb2b:GFP)Amaya NXR_1092 Description: (NBT/Tubb2-GFP) N-tubulin promoter driving GFP. GFP expression in neural tissue. ڱԳ:Xenbase. Xtr.Tg(vim:Venus;cryga:GFP)Amaya NXR_1093 Description: (Vimentin-GFP) Vimentin promoter driving Venus GFP, γ-cry driving GFP marker. GFP expression labels the deep cells of the neurectoderm and neural progenitors. GFP expression in lens. ڱԳ:Xenbase. Xtr.Tg(WntREs:dEGFP)Vlemx NXR_1094 Description: (pbin7LEF-GFP/Wnt Reporter)GFP expression reveals dynamic patterns of Wnt/beta-catenin signaling activity. The inserted transgene construct contains a synthetic Wnt-responsive promoter consisting of 7 copies of a TCF/LEF1 binding DNA element, a minimal TATA box and a reporter gene encoding destabilized eGFP and a polyA sequence. The transgene is flanked on both sides by two copies of the chicken HS4-core sequence. ڱԳ: Xtr.Tg(pax6:PR-Gal4-VP16;cryga:eCFP)Zimml NXR_1098 Description:(Pax6galPRBicolorBlue)Pax6 promoter driving RU486-inducible progesterone receptor/VP16/Gal4 transactivator, in transgene marked by g-cry ECFP cassette.ECFP in lens of eye; inducible Gal4 drives expression of UAS-transgenes in the presence of RU486 in the eye and dorsal CNS.Labels lens of eye; other functions depend on separate effector UAS transgenes ڱԳ: Xtr.Tg(tubb2b:Hsa.ALPP;cryga:dsRed)Amaya NXR_1099 Description: (NBT-PLAPxcryRFP) Neural expression of human placental alkaline phosphatase (ALPP) driven by N-beta-tubulin (tubb2b) upstream region. dsRed expression in eye from gamma crystallin (cryga) promoter used as marker. ڱԳ:. Xtr.Tg(Hsa.UBC.:lyn-memVenus)Amaya NXR_1106 Description: (UbiC-membraneVenus) Human Ubiquitin C (Hsa.UBC) promoter driving expression of a lyn kinase membrane domain fused to venus GFP. Ubiquitous Venus expression of cell membrane. ڱԳ:. Xtr.Tg(pax6:GFP)Amaya NXR_1107 Description: (Pax6-GFP) 3.6kb PAX6 promoter driving GFP. GFP expression in anterior and posterior neural plate plate. ڱԳ:. Xtr.Tg(UbiC Flox RFPGFP) NXR_1108 Description: Ubiquitous expression, floxed RFP GFP reporter. Ubiquitously expressed RFP; upon cre-medmiated recombination expresses GFP and loses RFP. Reference: Transgenic generated by Bettina Ryll. Xtr.Tg(Ntub GalPR BicB) NXR_1109 Description: (Ntub GalPR BicB) Ntub Galpr Bic- N-tubulin promoter driving Gal4Pr (RU482-inducible Gal4-progesterone receptor VP16 fusion) in post-mitotic neurons. Cry-CFP marker cassette (linked) ڱԳ: Xtr.Tg(Hsa.UBC:Gal4-VP16;cryga:CFP)Amaya NXR_1110 Description: (UbiqGal4,cryRFP) Human Ubiquitin C (UBC) promoter driving Gal4-VP16 fusion construct, with crystallin CFP marker. ڱԳ: Xtr.Tg(Dre.mxn1:GFP)Dorey NXR_1111 Description: (zHB9-GFP) Zebrafish HB9 promoter driving GFP. GFP expression in motorneurons and kidney. ڱԳ: Xtr.Tg(UAS:msn-mCherry;cryga:RFP)Amaya NXR_1117 Description: (UASmCherry-Moesin) UUAS driving mCherry-Moesin, γ-cry driving RFP marker. Labels F-actin. ڱԳ: Xtr.Tg(tubb2b:GCaMP6s,Rno.elas:GFP)NXR NXR_1123 Description: (ElasGFP:Tubb2-GCaMP6s) Neaural expression of the green Ca2+ sensor, GCaMP6s, and expression of GFP in the pancreas Reference: Custom transgenic generated by the NXR.